名古屋市立大学脳神経科学研究所

SAWAMOTO LAB 澤本研究室 - 名古屋市立大学医学研究科脳神経科学研究所 神経発達・再生医学分野

SAWAMOTO LAB 澤本研究室 - 名古屋市立大学医学研究科脳神経科学研究所 神経発達・再生医学分野

成体神経前駆細胞の増殖シグナルに関する新しい論文が掲載されました。A new paper published in Stem Cells Journals

成体神経前駆細胞の増殖シグナルに関する研究の成果がStem Cells Jpurnalsに掲載されました。

Adachi K, Mirzadeh Z, Sakaguchi M, Yamashita T, Nikolcheva T, Gotoh Y, Peltz G, Gong L, Kawase T, Alvarez-Buylla A, Okano H, Sawamoto K

Beta-catenin signaling promotes proliferation of progenitor cells in the adult mouse subventricular zone. 

Stem Cells 25:2827-2836 (2007)

筆頭著者による研究内容の解説はこちら

Stem Cells. 2007 Aug 2; PMID: 17673525

β-catenin signaling promotes proliferation of progenitor cells in the adult mouse subventricular zone

Kazuhide Adachi, Zaman Mirzadeh, Masanori Sakaguchi, Toru Yamashita, Tania Nikolcheva, Yukiko Gotoh, Gary Peltz, Leyi Gong, Takeshi Kawase, Arturo Alvarez-Buylla, Hideyuki Okano, Kazunobu Sawamoto

Abstract

The subventricular zone (SVZ) is the largest germinal zone in the mature rodent brain, and it continuously produces young neurons that migrate to the olfactory bulb. Neural stem cells in this region generate migratory neuroblasts via highly proliferative transit-amplifying cells. The Wnt/β-catenin signaling pathway partially regulates the proliferation and neuronal differentiation of neural progenitor cells in the embryonic brain. Here, we studied the role of beta-catenin signaling in the adult mouse SVZ. β-catenin-dependent expression of a destabilized form of green fluorescent protein was detected in progenitor cells in the adult SVZ of Axin2-d2EGFP reporter mice. Retrovirus-mediated expression of a stabilized β-catenin promoted the proliferation of Mash1+ cells and inhibited their differentiation into neuroblasts. Conversely, the expression of Dkk1, an inhibitor of Wnt signaling, reduced the proliferation of Mash1+ cells. In addition, an inhibitor of GSK3 promoted the proliferation of Mash1+ cells, and increased the number of new neurons in the olfactory bulb 14 days later. These results suggest that β-catenin signaling plays a role in the proliferation of progenitor cells in the SVZ of the adult mouse brain.

Key Words. β-catenin, neurogenesis, cell proliferation, subventricular zone, olfactory bulb, Mash1

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澤本研究室

SAWAMOTO LAB

Department of Developmental and Regenerative Neurobiology
Institute of Brain Science
Nagoya City University Graduate School of Medical Sciences

1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan

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